UG Anti-c-Abl (Ab-3) Mouse mAb (24-21)

Analysis Note

Positive ControlK562 (human bcr/abl), HL-60 (normal abl), and ANN-1 (murine abl) cells

Application

Immunoblotting (1-5 µg/ml)r>Immunofluorescence (see application references)r>Immunoprecipitation (1-2 µg/sample)r>Neutralization Studies (not recommended)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins.

Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation.

Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity.Antibody Target Gene Symbol: ABL1 Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase Hu Entrez ID: 25 (Related Antibodies: PK1006PK1013OP19) Mu Entrez ID: 11350 Rat Entrez ID: 311860

Immunogen

a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wiedemann, L.M., et al. 1988. Blood71, 349.Stam, K., et al. 1985. N. Engl. J. Med.313, 1429.Konopka, J.B., et al. 1984. Cell37, 1035.Prywes, R., et al. 1983. Cell34, 569.de Klein, A., et al. 1982. Nature300, 765.Reynolds, F.H., Jr., et al. 1980. J. Virol.36, 374.Reynolds, F.H., Jr., et al. 1978. Proc. Natl. Acad. Sci. USA75, 3974.Witte, O.N., et al. 1978. Proc. Natl. Acad. Sci. USA75, 2488.Abelson, H.T. and Rabstein, L.S. 1970. Cancer Res.30, 2213.

Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)